After the course, the students will be able to:
- Reflect on the different classes of bio-inks and the characteristics which a bio-ink should have
- Analyze the requirements for the successful extrusion of living cells
- Consider and apply tissue remodeling within the concept of 4D bioprinting
- Understand the conceptual potentials and limitations of 3D bioprinting
- Theorize current challenges and potential solutions in 3D bioprinting
- Design a project plan to address a current limitation in the field
As with all areas of additive manufacturing, 3D bioprinting has seen tremendous progress and developments in the last decade. 3D bioprinting combines advanced 3D fabrication techniques with biological systems to create designed tissue constructs, which can be applied for tissue engineering, as 3D in vitro biological models, or for other applications. This course will provide the student with fundamental insights on important aspects of 3D bioprinting, with a particular emphasis on extrusion bioprinting. Topics include bio-ink development, rheology of 3D bioprinting, inclusion of cells and 4D bioprinting, tissue engineering and translation, biohybrid robotics, and ethics.
- The content that is covered in the course includes:
- Introduction, different methodologies for 3D biofabrication, from medical imaging to G-code to print, complexity in tissues
- Current limitations and main challenges in tissue engineering and 3D biofabrication
- Bio-inks; tissue derived matrices, biomaterial properties design, multiscale functional materials; crosslinking strategies
- Rheology of bio-inks, printability, embedded printing
- Inclusion of cells, tissue remodeling, 4D bioprinting
- Challenge lecture 1: write a 2-page proposal describing a new optimal 3D bioprinting procedure to produce a patterned construct with a complex shape.
- Tissue engineering, translation, in-clinic and in-vivo printing
- Biohybrid robotics
- Environmental, economic, and ethical implications of biofabrication
- Practical: Convert a filament printer into an extrusion hydrogel printer. Investigate the relationship between hydrogel rheological properties and printability.