Biophysical Techniques & Molecular Imaging (BT&MI) introduces a selection of advanced micro-spectroscopic techniques for molecular and cellular studies. Micro-spectroscopic techniques are essential to modern biomedical sciences, including quantitative biology, stem cell research and understanding of fundamental cellular processes.
The course focuses on imaging techniques based on fluorescence spectroscopy and vibrational spectroscopy. General concepts of various microscopies will be introduced, methods to probe molecular interaction, molecular motion, conformational dynamics from in vitro to the cellular level will be discussed. Concepts will be illustrated with examples from the life and (bio)-material sciences.
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Fundamentals fluorescence and vibrational transitions, related parameters; instrumentation spectroscopy and microscopy (widefield, confocal, fluorescrence, Raman); fluorophores (intrinsic, extrinsic, fluorescent proteins) and labeling strategies; monitoring molecular interactions (anisotropy, MST, FCS, quenching), molecular motion (FRAP, FLIP, FCS); single molecule spectroscopy; superresolution microscopy (single molecule localization, STED); accessing molecular structure (fluorescence based, electron microscopy)
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80% of grade: Written exam; needs to be passed with minimum grade of 5.5
20 % of grade: Study and presentation on a selected topic in advanced biophysics imaging when exam better than 5.5
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