Luminscence: Fluorescence, phosphorescence, bioluminescence; advanced luminescence principles: polarization, lifetime; bulk and single molecule approaches; imaging and spectroscopy in a microscope; intrinsic and extrinsic fluorophores; protein fluorescence; genetically encodable fluorescent markers, nanoparticles
Microscopy: wide-field, dark field, confocal, phase contrast, fluorescence microscopy (FRAP, FLIP, FLIM, FRET), micro-spectroscopy, hyperspectral imaging, polarization contrast, lifetime imaging, resonance energy transfer imaging, nano-particle imaging, non-linear microscopy.
Vibrational Spectroscopy and Imaging: label-free contrast methods such as spontaneous Raman micro-spectroscopy, Infrared microscopy, CARS microscopy, non-linear fluorescence microscopy and single molecule micro-spectroscopy.

Assesment:
Study and presentation on a selected topic in advanced biophysics imaging will contribute for 20% to the final grade.